DNA fingerprinting database to finger criminals

A DNA Fingerprinting Exercise for

"Hands on" laboratory exercises are excellent teaching tools because they have the potential to reach students with all types of learning styles. Unfortunately the large monetary investment required to obtain supplies and equipment and train instructors prevents many schools from providing hands-on learning experiences with DNA technology. Students in non-laboratory courses are even less likely...

DNA Fingerprinting and RFLP Analysis

DNA Fingerprinting of Pearls to Determine Their Origins

We report the first successful extraction of oyster DNA from a pearl and use it to identify the source oyster species for the three major pearl-producing oyster species Pinctada margaritifera, P. maxima and P. radiata. Both mitochondrial and nuclear gene fragments could be PCR-amplified and sequenced. A polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) assay in the i...

SysZNF: the C2H2 zinc finger gene database

C2H2 zinc finger (C2H2-ZNF) genes are one of the largest and most complex gene super-families in metazoan genomes, with hundreds of members in the human and mouse genome. The ongoing investigation of this huge gene family requires computational support to catalog genotype phenotype comparisons of C2H2-ZNF genes between related species and finally to extend the worldwide knowledge on the evoluti...

Automated Image Analysis for Dna Fingerprinting

Methods for automated detection of DNA restriction fragments resolved on agarose fingerprinting gels are described. We present a mathematical model for the location and shape of the restriction fragments as a function of fragment size, with model parameters determined empirically from marker lanes containing molecular size standards. Automated identification of restriction fragments involves se...